Email this article Low blood Pb levels could adversely affect the pro-oxidant balance in rat epididymides
Abstract
Materials and methods: The intensity of oxidative stress was measured by changes in lipid peroxidation (by chemiluminiscence method), as well as in protein expression and the activity of superoxide dismutase (SOD) and catalase (CAT). Additionally, it was investigated whether the toxic effects of Pb could be alleviated by the simultaneous administration of the antioxidants – tocopherol (vitamin E) or its water-soluble counterpart Trolox.
Results: Chronic exposure to Pb resulted in increased lipid peroxidation in the epididymis (manifested by a marked intensification in chemiluminescence from the caput and cauda epididymis). Significantly, when Pb-treated rats were also supplemented with Trolox or tocopherol, the increase in chemiluminiscence intensity was significantly lower, which indicated the beneficial effect of these antioxidants on reduction of Pb-induced oxidative stress. However, a direct beneficial effect of supplementation on the activity and expression of SOD and CAT was not demonstrated.The activity and expression of SOD was not affected by exposure to Pb or to simultaneous administration of antioxidants. The use of tocopherol and Trolox had also no beneficial effect on the Pb-induced reduction in the activity and expression of CAT.
Conclusions: The environmental Pb-exposure leading to metal accumulation in whole blood at oncentrations below the “threshold level” (5 μg/dL) may cause disruption in the prooxidant/antioxidant balance in rat epididymides containing epididymal spermatozoa.
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DOI: https://doi.org/10.21164/pomjlifesci.816
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