Investigation of the molecular alterations associated with the efficacy of microneedling therapy and its potential applications in medical and dental practices

Eryk Prajwos, Małgorzata Król, Kamil Janawa, Anna Surówka, Patrycja Kupnicka, Dariusz Chlubek

Abstract


Microneedling is an emerging minimally invasive technique that induces microscopic wounds in the skin or oral mucosa, stimulating healing and rejuvenation through complex biological processes. This review examines the impact of microneedling on inflammation, highlighting its role in fibroblasts activation, modulation of cytokine release, neovascularization, and the regulation of matrix metalloproteinases activity. By engaging these pathways, microneedling facilitates tissue remodeling and enhances the skin's regenerative capacity, rendering it beneficial for various conditions, such as wound healing, skin rejuvenation, and periodontal therapy. Recent technological advancements in microneedling, including its application for targeted drug delivery and incorporation of biologically active compounds, hold promise for future clinical use. Understanding the mechanisms underlying the therapeutic effects of microneedling can optimize treatment strategies, maximizing clinical outcomes while minimizing side effects.


Keywords


microneedling; inflammation; tissue regeneration

Full Text:

PDF

References


Rao KHS. How to practice dermatosurgery safely? J Cutan Aesthet Surg 2014;7(2):121-3. doi: 10.4103/0974-2077.138361.

Chu S, Foulad DP, Atanaskova Mesinkovska N. Safety profile for microneedling: a systematic review. Dermatol Surg 2021;47(9):1249-54. doi: 10.1097/01.DSS.0000790428.70373.f6.

Gowda A, Healey B, Ezaldein H, Merati M. A systematic review examining the potential adverse effects of microneedling. J Clin Aesthet Dermatol 2021;14(1):45-54.

Leatham H, Guan L, Chang ALS. Unintended widespread facial autoinoculation of varicella by home microneedling roller device. JAAD Case Rep 2018;4(6):546-7. doi: 10.1016/j.jdcr.2018.02.004.

Schmitt L, Marquardt Y, Amann P, Heise R, Huth L, Wagner-Schiffler S, et al. Comprehensive molecular characterization of microneedling therapy in a human three-dimensional skin model. PLoS One 2018;13(9):e0204318. doi: 10.1371/journal.pone.0204318.

Guo Q, Su W, Wen F, Cai J, Huo L, Zhong H, et al. α-Amylase and polydopamine@polypyrrole-based hydrogel microneedles promote wound healing by eliminating bacterial infection. Int J Biol Macromol 2024;281(Pt 4):136604. doi: 10.1016/j.ijbiomac.2024.136604.

Lyu J, Jiang L, Tang M, Zheng L, Liu S. Microneedle fractional radiofrequency associated with drug delivery for facial atrophic acne scars and skin rejuvenation. J Cosmet Laser Ther 2024;26(1-4):61-8. doi: 10.1080/14764172.2024.2372342.

Dhurat R, Sukesh M, Avhad G, Dandale A, Pal A, Pund P. A randomized evaluator blinded study of effect of microneedling in androgenetic alopecia: a pilot study. Int J Trichology 2013;5(1):6-11. doi: 10.4103/0974-7753.114700.

Cao W, Xing H, Guo S, Wang L, Sui X, Huang L, et al. Aspirin in the form of microneedle repairs DNA and reduces inflammation in persistent skin damage. Biomater Res 2024;28:0083. doi: 10.34133/bmr.0083.

Luo X, Yang L, Cui Y. Microneedles: materials, fabrication, and biomedical applications. Biomed Microdevices 2023;25(3):20. doi: 10.1007/s10544-023-00658-y.

Li Y, Bi D, Hu Z, Yang Y, Liu Y, Leung WK. Hydrogel-forming microneedles with applications in oral diseases management. Materials (Basel) 2023;16(13):4805. doi: 10.3390/ma16134805.

Khan MUA, Aslam MA, Abdullah MFB, Gul H, Stojanović GM, Abdal-Hay A, et al. Microneedle system for tissue engineering and regenerative medicines: a smart and efficient therapeutic approach. Biofabrication 2024;16(4). doi: 10.1088/1758-5090/ad6d90.

Ferreira LEN, Franz-Montan M, Benso B, Gill HS. Microneedles for oral mucosal delivery – Current trends and perspective on future directions. Expert Opin Drug Deliv 2023;20(9):1251-65. doi: 10.1080/17425247.2023.2264189.

Lima E, Lima M, Takano D. Microneedling: experimental study and classification of the resulting injury. Surg Cosmet Dermatol 2013;5(2):110-4.

Sasaki GH. Micro-needling depth penetration, presence of pigment particles, and fluorescein-stained platelets: clinical usage for aesthetic concerns. Aesthet Surg J 2017;37(1):71-83. doi: 10.1093/asj/sjw120.

Mostafa D, Alarawi R, AlHowitiy S, AlKathiri N, Alhussain R, Almohammadi R, et al. The effectiveness of microneedling technique using coconut and sesame oils on the severity of gingival inflammation and plaque accumulation: A randomized clinical trial. Clin Exp Dent Res 2022;8(5):1249-58. doi: 10.1002/cre2.618.

P SM, S S. Effectiveness of microneedling technique using olive oil on the severity of gingival inflammation and plaque accumulation: a randomised controlled trial. Cureus 2024;16(4):e59415. doi: 10.7759/cureus.59415.

Zhang X, Wang Y, Chi J, Zhao Y. Smart microneedles for therapy and diagnosis. Research (Wash D C) 2020;2020:7462915. doi: 10.34133/2020/7462915.

Chen Y, Alba M, Tieu T, Tong Z, Minhas RS, Rudd D, et al. Engineering micro-nanomaterials for biomedical translation. Adv NanoBiomed Res 2021;1(9):2100002. doi: 10.1002/anbr.202100002.

Hamed R, Abu Nahia BJ, Alkilani AZ, Al-Adhami Y, Obaidat R. Recent advances in microneedling-assisted cosmetic applications. Cosmetics 2024;11(2):51. doi: 10.3390/cosmetics11020051.

Das S, Baker AB. Biomaterials and nanotherapeutics for enhancing skin wound healing. Front Bioeng Biotechnol 2016;4:82. doi: 10.3389/fbioe.2016.00082.

Ricciotti E, FitzGerald GA. Prostaglandins and inflammation. Arterioscler Thromb Vasc Biol 2011;31(5):986-1000. doi: 10.1161/ATVBAHA.110.207449.

Soliman AM, Barreda DR. Acute inflammation in tissue healing. Int J Mol Sci 2022;24(1):641. doi: 10.3390/ijms24010641.

Serhan CN, Brain SD, Buckley CD, Gilroy DW, Haslett C, O’Neill LAJ, et al. Resolution of inflammation: state of the art, definitions and terms. FASEB J 2007;21(2):325-32. doi: 10.1096/fj.06-7227rev.

Chen L, Deng H, Cui H, Fang J, Zuo Z, Deng J, et al. Inflammatory responses and inflammation-associated diseases in organs. Oncotarget 2017;9(6):7204-18. doi: 10.18632/oncotarget.23208.

Sugimoto MA, Sousa LP, Pinho V, Perretti M, Teixeira MM. Resolution of inflammation: what controls its onset? Front Immunol 2016;7:160. doi: 10.3389/fimmu.2016.00160.

Gomes RN, Manuel F, Nascimento DS. The bright side of fibroblasts: molecular signature and regenerative cues in major organs. NPJ Regen Med 2021;6(1):43. doi: 10.1038/s41536-021-00153-z.

Page MJ, McKenzie JE, Bossuyt PM, Boutron I, Hoffmann TC, Mulrow CD, et al. The PRISMA 2020 statement: an updated guideline for reporting systematic reviews. BMJ 2021;372:n71. doi: 10.1136/bmj.n71.

Bennett NT, Schultz GS. Growth factors and wound healing: biochemical properties of growth factors and their receptors. Am J Surg 1993;165(6):728-37. doi: 10.1016/s0002-9610(05)80797-4.

You L, Yang C, Du Y, Wang W, Sun M, Liu J, et al. A systematic review of the pharmacology, toxicology and pharmacokinetics of matrine. Front Pharmacol 2020;11:01067. doi: 10.3389/fphar.2020.01067.

Moreira HR, Marques AP. Vascularization in skin wound healing: where do we stand and where do we go? Curr Opin Biotechnol 2022;73:253-62. doi: 10.1016/j.copbio.2021.08.019.

Baris R, Kankaya Y, Ozer K, Kocer RG, Bektas CI, Karatas A, et al. The effect of microneedling with a roller device on the viability of random skin flaps in rats. Plast Reconstr Surg 2013;131(5):1024-34. doi: 10.1097/PRS.0b013e3182879edf.

Cao Y, Chen B, Liu Q, Mao Y, He Y, Liu X, et al. Dissolvable microneedle--based wound dressing transdermally and continuously delivers anti-inflammatory and pro-angiogenic exosomes for diabetic wound treatment. Bioact Mater 2024;42:32-51. doi: 10.1016/j.bioactmat.2024.08.016.

Strizova Z, Benesova I, Bartolini R, Novysedlak R, Cecrdlova E, Foley LK, et al. M1/M2 macrophages and their overlaps – myth or reality? Clin Sci (Lond) 2023;137(15):1067-93. doi: 10.1042/CS20220531.

Gill SE, Parks WC. Metalloproteinases and their inhibitors: Regulators of wound healing. Int J Biochem Cell Biol 2008;40(6-7):1334-47. doi: 10.1016/j.biocel.2007.10.024.

de Almeida LGN, Thode H, Eslambolchi Y, Chopra S, Young D, Gill S, et al. Matrix metalloproteinases: from molecular mechanisms to physiology, pathophysiology, and pharmacology. Pharmacol Rev 2022;74(3):712-68. doi: 10.1124/pharmrev.121.000349.

Liu X, Barresi R, Kaminer M, Qian K, Thillou F, Bataillon M, et al. Utilization of ex vivo tissue model to study skin regeneration following microneedle stimuli. Sci Rep 2022;12(1):18115. doi: 10.1038/s41598-022-22481-w.

Keskin ES, Keskin ER, Öztürk MB, Çakan D. The effect of MMP-1 on wound healing and scar formation. Aesthetic Plast Surg 2021;45(6):2973-9. doi: 10.1007/s00266-021-02369-2.

Singh D, Rai V, Agrawal DK. Regulation of collagen I and collagen III in tissue injury and regeneration. Cardiol Cardiovasc Med 2023;7(1):5-16. doi: 10.26502/fccm.92920302.

Lopes J, Adiguzel E, Gu S, Liu SL, Hou G, Heximer S, et al. Type VIII collagen mediates vessel wall remodeling after arterial injury and fibrous cap formation in atherosclerosis. Am J Pathol 2013;182(6):2241-53. doi: 10.1016/j.ajpath.2013.02.011.

Vaalamo M, Leivo T, Saarialho-Kere U. Differential expression of tissue inhibitors of metalloproteinases (TIMP-1, -2, -3, and -4) in normal and aberrant wound healing. Hum Pathol 1999;30(7):795-802. doi: 10.1016/s0046-8177(99)90140-5.

Omolu A, Bailly M, Day RM. Assessment of solid microneedle rollers to enhance transmembrane delivery of doxycycline and inhibition of MMP activity. Drug Deliv 2017;24(1):942-51. doi: 10.1080/10717544.2017.1337826.

Fabbrocini G, Fardella N, Monfrecola A, Proietti I, Innocenzi D. Acne scarring treatment using skin needling. Clin Exp Dermatol 2009;34(8):874-9. doi: 10.1111/j.1365-2230.2009.03291.x.

Chen Y, Ma L, Cheng Z, Hu Z, Xu Y, Wu J, et al. Senescent fibroblast facilitates re-epithelization and collagen deposition in radiation-induced skin injury through IL-33-mediated macrophage polarization. J Transl Med 2024;22(1):176. doi: 10.1186/s12967-024-04972-8.

Zhang Q, Shi L, He H, Liu X, Huang Y, Xu D, et al. Down-regulating scar formation by microneedles directly via a mechanical communication pathway. ACS Nano 2022;16(7):10163-78. doi: 10.1021/acsnano.1c11016.

Zhang Q, Shi L, He H, Liu X, Huang Y, Xu D, et al. Correction to “Down--regulating scar formation by microneedles directly via a mechanical communication pathway”. ACS Nano 2023;17(11):11070-1. doi: 10.1021/acsnano.3c03389.

Qi F, Xu Y, Zheng B, Li Y, Zhang J, Liu Z, et al. The core-shell microneedle with probiotic extracellular vesicles for infected wound healing and microbial homeostasis restoration. Small 2024;20(46):e2401551. doi: 10.1002/smll.202401551.

Zaaya S, Elbattawy W, Yusri S, Fawzy El-Sayed KM. Micro-needling versus acellular dermal matrix in RT1 gingival recession coverage: A randomized clinical trial. J Periodontal Res 2024;59(5):907-17. doi: 10.1111/jre.13271.

Checchi V, Maravic T, Bellini P, Generali L, Consolo U, Breschi L, et al. The role of matrix metalloproteinases in periodontal disease. Int J Environ Res Public Health 2020;17(14):4923. doi: 10.3390/ijerph17144923.

Lee H, Min HS, Jang M, Kang G, Gong S, Lee C, et al. Lidocaine-loaded dissolving microneedle for safe local anesthesia on oral mucosa for dental procedure. Expert Opin Drug Deliv 2023;20(6):851-61. doi: 10.1080/17425247.2023.2216450.

Yang M, Pan L, Tian H, Zhou T, Xin H, Feng Y, et al. pH- and matrix metalloproteinase-responsive multifunctional bilayer microneedles platform for treatment of tinea pedis. ACS Biomater Sci Eng 2024;10(5):3108-19. doi: 10.1021/acsbiomaterials.4c00305.

Liu X, Diao N, Song S, Wang W, Cao M, Yang W, et al. Inflammatory macrophage reprogramming strategy of fucoidan microneedles-mediated ROS-responsive polymers for rheumatoid arthritis. Int J Biol Macromol 2024;271(Pt 2):132442. doi: 10.1016/j.ijbiomac.2024.132442.

Yuan J, Yang H, Liu C, Shao L, Zhang H, Lu K, et al. Microneedle patch loaded with exosomes containing microRNA-29b prevents cardiac fibrosis after myocardial infarction. Adv Healthc Mater 2023;12(13):e2202959. doi: 10.1002/adhm.202202959.

Shih T, Lindley C. Bevacizumab: an angiogenesis inhibitor for the treatment of solid malignancies. Clin Ther 2006;28(11):1779-802. doi: 10.1016/j.clinthera.2006.11.015.

Kim YC, Grossniklaus HE, Edelhauser HF, Prausnitz MR. Intrastromal delivery of bevacizumab using microneedles to treat corneal neovascularization. Invest Ophthalmol Vis Sci 2014;55(11):7376-86. doi: 10.1167/iovs.14-15257.

Singh A, Yadav S. Microneedling: Advances and widening horizons. Indian Dermatol Online J 2016;7(4):244-54. doi: 10.4103/2229-5178.185468.

Batra P, Dawar A, Miglani S. Microneedles and nanopatches-based delivery devices in dentistry. Discoveries (Craiova) 2020;8(3):e116. doi: 10.15190/d.2020.13.




DOI: https://doi.org/10.21164/pomjlifesci.1109

Copyright (c) 2024 Eryk Prajwos, Małgorzata Król, Kamil Janawa, Anna Surówka, Patrycja Kupnicka, Dariusz Chlubek

License URL: https://creativecommons.org/licenses/by-nc-nd/3.0/pl/