Allele frequency distribution and forensic parameters of 23 autosomal STR loci in the Polish population using the PowerPlex® Fusion 6C System
DOI:
https://doi.org/10.21164/pomjlifesci.1224Keywords:
short tandem repeats, PowerPlex® Fusion 6C, allele frequency, forensic geneticsAbstract
Introduction: Databases of autosomal STR marker allele frequencies are a key tool in forensic genetics, enabling accurate calculations of kinship probability or DNA profile matching. The available data for the Polish population mainly concern older marker sets. There are no comprehensive publications covering the full panel of 23 autosomal loci of the PowerPlex® Fusion 6C system.
The aim of this study is to present the current allele frequencies for 23 autosomal loci from PowerPlex® Fusion 6C set in the Polish population.
Materials and methods: A total of 1043 genetic profiles obtained from buccal swabs collected from individuals of Polish origin were analyzed. Amplification was performed with the PowerPlex® Fusion 6C kit, and electropherogram analysis was conducted using GeneMapper® ID-X v1.4 software. Statistical analyses were carried out with Familias v. 3.4, Arlequin v. 3.5 and Forstat software.
Results: Observed heterozygosity (Ho) values ranged from 0.5973 (TPOX) to 0.9588 (SE33). The highest power of discrimination (PD) was observed for SE33 (PD = 0.9939), Penta E (PD = 0.9812), and D1S1656 (PD = 0.9809). All loci met Hardy-Weinberg equilibrium criteria. The combined power of discrimination (CPD) was >0.99999999999999999999, and the combined power of exclusion (CPE) was 0.999999999967.
Conclusions: The obtained results confirm the high analytical value of the PowerPlex® Fusion 6C system for forensic and kinship testing. The developed allele frequency database represents a valuable reference tool for the Polish population.
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