Email this article Determination of fentanil and remifentanil in blood with liquid chromatography coupled with tandem mass spectrometry LC-MS/MS
Abstract
Introduction: Fentanil and remifentanil are both opioid drugs. As synthetic compounds they act as agonists on the opioid brain receptors. They are widely used in neuroleptanalgesia as well as in various clinical procedures. Due to their wide applicability, there is a need to develop appropriate toxicological assays for qualitative determination and to quantify the estimation of fentanil and remifentanil in biological material.
The aim of this study was to develop a method for the parallel qualitative and quantitative determination of fentanil and remifentanil in whole blood with the determination of analytical limit parameters.
Materials and methods: Biological material in the above study was whole blood collected from the Blood Donation Station in Szczecin, Poland. Samples for tests were obtained by adding reference substances (fentanil, remifentanil, fentanil-d5 with known concentrations) to whole blood. Analytes were isolated from the matrix by liquid-liquid extraction with ethyl acetate. The obtained samples were further analyzed by high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). The following parameters were calculated based on measurements: mean, standard deviation, relative standard deviation, and coefficient of variation. To increase the sensitivity of the LC-MS/MS method and to optimize the procedure a potential range over 0–300 V and DP = 80 was chosen. Collision energy was 35 V. The high resolution of the apparatus allowed the selection of a narrow signal range for each compound: fentanil-d5 – 188.143 ±0.0047 Da, fentanil – 188.1445 ±0.0043 Da and remifentanil – 317.186 ±0.007 Da.
Results: The results obtained from the analysis of fentanil and remifentanil samples allowed for plotting calibration curves within the range of 1–200 ng/mL, which showed linearity.
Conclusion: The obtained results allowed the identification and the quantitative determination of fentanil and remifentanil in whole blood. Liquid chromatography-tandem mass spectrometry is suitable for practical applications in both forensic and clinical toxicology.
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DOI: https://doi.org/10.21164/pomjlifesci.439
Copyright (c) 2018 Tomasz Janus, Barbara Potocka-Banaś, Ewa Jasionowicz, Krzysztof Borowiak, Izabela Polewczak
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